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Category:Computational musical instruments
Category:Digital signal processingABSTRACT
Pipet-based biochip has been an important tool for the high throughput screening (HTS). However, existing biochip technology is still developing and employing new techniques to increase its throughput. Although high-throughput biochips have been developed, they require complex chip fabrication process which causes an increase in production cost and time. In this paper, we propose a microfluidic chip with a simple step-by-step fabrication process, which makes the biochip process fast and affordable. The technique may also benefit on-site HTS of drug discovery. The proposed method is a simple procedure that has been divided into three steps: 1) channel formation, 2) membrane formation and 3) biochip formation. The platform is equipped with microchannels and channels for sample introduction, biochip formation and molecule delivery. The biochip consists of a 48×48 array of microchannels. Two different methods have been employed to fabricate microchannels in the biochip. Both methods are a direct patterning of poly (methyl methacrylate) (PMMA) on silicon substrate and sandwich structure of agarose. Using this method, it is possible to fabricate an array of microchannels in one step. The biochip shows high throughput real-time HTS of antibody-antigen interaction and antigen-epitope interaction. In addition, two ELISA kits have been developed using two different biochips. One is for the detection of the binding between native proteins and antibodies using target DNA as a sample and the other is for the detection of the immune complex of a native protein and an antibody using the biotinylated antibody as a sample. The proposed method can be used for various biochips as a platform of drug discovery.
pone.0138547.g001: Development of microfluidic chip. The procedure for fabricating a microfluidic chip consists of three parts. (a) Preparation of microwell arrays, (b) patterning of PMMA for chip formation and (c) sandwich structure agarose for biochip.Fig 1
pone.0138547.g002: Prototype of a microfluidic chip for HTS. The microfluidic chip is equipped with (a) microwells and microchannels for (b) reagent delivery and (c) microchannel for sample introduction.Fig 2
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