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We describe a novel liposome formulation with liposome coated with a pH-responsive polymer using microemulsion technology. The formulation was designed to release a drug from the liposome at the target site. The liposomes were prepared by the dehydration-rehydration method using soybean phosphatidylcholine (PC). The pH-responsive polymer was synthesized using a pH-sensitive acrylate comonomer and a pH-responsive monomer. The formulation contained a drug, a fluorophore (rhodamine-dextran), and the pH-responsive polymer (pH-responsive polymer-PC/liposome). The aim of the present study is to design and characterize the novel pH-responsive polymer-coated liposomes for drug delivery. The release of the drug from the liposomes coated with the pH-responsive polymer was visualized by confocal laser scanning microscopy. This simple technique has practical advantages for in vivo drug release. The results showed that the rhodamine fluorescence intensity increased as a function of pH, and the drug release was clearly visualized as a function of time by confocal laser scanning microscopy. From our results, we concluded that the novel liposome formulation can provide a suitable environment for drug delivery in the target site with pH-responsive drug release.

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