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A catecholamine with the unusual [2.2.1] bicyclic octahydronaphthalene system was prepared from cyclohexanone using a novel Pinner reaction. The synthesized compound, cymbopetaline oteropone (CPTP), potently inhibited growth of rat C6 glioma cells in culture with an IC50 of 50 nM. This effect was accompanied by necrosis in the C6 glioma cells as demonstrated by the formation of dark red turbid zones in the culture plates. The strong cytotoxic effect of CPTP was observed regardless of the cell number used in the assay, and the cytotoxic effect was unlikely to be due to nonspecific toxicity. Treatment of C6 glioma cells with 1 microM CPTP resulted in a marked decrease in catecholamine synthesis, which lasted up to 48 hr. This effect was accompanied by a marked decrease in tyrosine hydroxylase activity in the tissue (approximately 90% of control), which was partially reversed by co-incubation with 1 microM CPTP. However, the activity of choline acetyltransferase in tissue homogenates was unaffected. It was apparent that the potent cytotoxicity of CPTP was not due to irreversible inhibition of tyrosine hydroxylase activity.up, getting ready to move to Spain, and I swear, someone or a child of someone actually did.
I wouldn’t have guessed so, but it seems there was an uprising in the UK that overthrew the Queen.
Indeed, but the British people are smart. They’re able to recognize the value of money and are fond of their food, so this might have been enough
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