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counter strike source 2.5 crack indir. World Wide Web. www. Evaluation of direct PCR for diagnosis of Helicobacter pylori in mucosa.
Polymerase chain reaction (PCR) has been used for Helicobacter pylori detection but suffers from low sensitivity and requires multiple nested PCR analyses for identification of strains. Furthermore, about 30% of H. pylori strains are potentially resistant to metronidazole or clarithromycin. We evaluated direct PCR for H. pylori detection in gastric biopsies. DNA was extracted from the biopsies and purified by the EZNA tissue kit (Qiagen, Hilden, Germany). We designed primer pairs at the conserved part of the 16S rRNA gene of H. pylori. PCR was done by the HotStarTaq Plus Master Mix Kit (Qiagen). Samples from 58 patients, 20 with and 38 without H. pylori infection, were analyzed. The direct PCR produced positive results in 17.2% (4/23) of H. pylori infected biopsies. The sensitivity of the direct PCR was 14.3% and the specificity was 100%. The false negative results were obtained in patients with acute gastritis. The results of direct PCR for H. pylori detection in gastric bi https://handelsweb.be/wp-content/uploads/2022/06/halialli.pdf
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Nov 7, 2019
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